Sample Volumes
The sample volume can vary greatly depending on the type of toxicity testing being performed and the number of species being tested. NEB uses 1 liter, 1 gallon, 2.5 gallon and 5 gallon sized cubitainers.
| Test Type | Effluent Volume | Receiving Water Volume |
| 2 species freshwater chronic | Sample #1: 2.5 gallon Sample #2: 2.5 gallon Sample #3: 5 gallons | Sample #1: 5 gallons Sample #2: 5 gallons Sample #3: 7.5 gallons |
| 2 species saltwater chronic | Sample #1: 5 gallons Sample #2: 5 gallons Sample #3: 7.5 gallons | Sample #1: 10 gallons Sample #2: 10 gallons Sample #3: 15 gallons |
| Ceriodaphnia only chronic | Sample #1: 2.5 gallon Sample #2: 1 gallon Sample #3: 1 gallon | Sample #1: 2.5 gallon Sample #2: 1 gallon Sample #3: 2.5 gallon |
| 2 species freshwater acute | 2.5 gallons | 2.5 gallons |
| 2 species saltwater acute | 2.5 gallons | 5 gallons |
| 2 species freshwater pass/fail acute | 1 gallon | N/A |
| 2 species saltwater pass/fail acute | 2.5 gallons | N/A |
| Connecticut D.pulex Stormwater acute | 1 liter | N/A |